Post-weaning diarrhea (PWD) and associated mortality in commercial swine production remain a significant challenge for antibiotic-free meat production. With the ban on antibiotic usage in livestock production, probiotics have gained a lot of attention as a potential alternative. However, strain and host specificity limit their efficacy. In this research, I tested probiotic potential of a novel Bacillus subtilis, BS9 and its impact on growth performance and gut health of weaning piglets.
Three studies were conducted. In the first study, the influence of a simulated digest of a dietary supplement on the contractile response of gastric smooth muscle to acetylcholine (Ach) was evaluated. Porcine gastric smooth muscle was collected and established in organ baths with either a simulated digest of the supplement (FA), a blank digest with no supplement (BL), or a PBS control (CO). Increasing concentrations of Ach were added and contractile force measured using an isometric force transducer.